NBP1-89113

antibody from Novus Biologicals

Targeting: FUS ALS6, FUS1, hnRNP-P2, HNRNPP2, TLS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89113

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89113, RRID:AB_11026765

Product name

Rabbit Polyclonal FUS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human FUS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Amelioration of toxicity in neuronal models of amyotrophic lateral sclerosis by hUPF1.

Barmada SJ, Ju S, Arjun A, Batarse A, Archbold HC, Peisach D, Li X, Zhang Y, Tank EM, Qiu H, Huang EJ, Ringe D, Petsko GA, Finkbeiner S
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 23;112(25):7821-6

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Neuropathologic analysis of Tyr69His TTR variant meningovascular amyloidosis with dementia.

Ziskin JL, Greicius MD, Zhu W, Okumu AN, Adams CM, Plowey ED
Acta neuropathologica communications 2015 Jul 10;3:43

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Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease.

Nakamura M, Murray ME, Lin WL, Kusaka H, Dickson DW
American journal of neurodegenerative disease 2014;3(2):93-102

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Activity-dependent FUS dysregulation disrupts synaptic homeostasis.

Sephton CF, Tang AA, Kulkarni A, West J, Brooks M, Stubblefield JJ, Liu Y, Zhang MQ, Green CB, Huber KM, Huang EJ, Herz J, Yu G
Proceedings of the National Academy of Sciences of the United States of America 2014 Nov 4;111(44):E4769-78

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ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects.

Qiu H, Lee S, Shang Y, Wang WY, Au KF, Kamiya S, Barmada SJ, Finkbeiner S, Lui H, Carlton CE, Tang AA, Oldham MC, Wang H, Shorter J, Filiano AJ, Roberson ED, Tourtellotte WG, Chen B, Tsai LH, Huang EJ
The Journal of clinical investigation 2014 Mar;124(3):981-99

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Immunoreactivity of valosin-containing protein in sporadic amyotrophic lateral sclerosis and in a case of its novel mutant.

Ayaki T, Ito H, Fukushima H, Inoue T, Kondo T, Ikemoto A, Asano T, Shodai A, Fujita T, Fukui S, Morino H, Nakano S, Kusaka H, Yamashita H, Ihara M, Matsumoto R, Kawamata J, Urushitani M, Kawakami H, Takahashi R
Acta neuropathologica communications 2014 Dec 10;2:172

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Frontotemporal dementia-amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1-q12.2: genetic, clinical and neuropathological analysis.

Dobson-Stone C, Luty AA, Thompson EM, Blumbergs P, Brooks WS, Short CL, Field CD, Panegyres PK, Hecker J, Solski JA, Blair IP, Fullerton JM, Halliday GM, Schofield PR, Kwok JB
Acta neuropathologica 2013 Apr;125(4):523-33

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Arginine methylation next to the PY-NLS modulates Transportin binding and nuclear import of FUS.

Dormann D, Madl T, Valori CF, Bentmann E, Tahirovic S, Abou-Ajram C, Kremmer E, Ansorge O, Mackenzie IR, Neumann M, Haass C
The EMBO journal 2012 Nov 14;31(22):4258-75

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Distinct clinical and pathological characteristics of frontotemporal dementia associated with C9ORF72 mutations.

Snowden JS, Rollinson S, Thompson JC, Harris JM, Stopford CL, Richardson AM, Jones M, Gerhard A, Davidson YS, Robinson A, Gibbons L, Hu Q, DuPlessis D, Neary D, Mann DM, Pickering-Brown SM
Brain : a journal of neurology 2012 Mar;135(Pt 3):693-708

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FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations.

Neumann M, Bentmann E, Dormann D, Jawaid A, DeJesus-Hernandez M, Ansorge O, Roeber S, Kretzschmar HA, Munoz DG, Kusaka H, Yokota O, Ang LC, Bilbao J, Rademakers R, Haass C, Mackenzie IR
Brain : a journal of neurology 2011 Sep;134(Pt 9):2595-609

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A comparative clinical, pathological, biochemical and genetic study of fused in sarcoma proteinopathies.

Lashley T, Rohrer JD, Bandopadhyay R, Fry C, Ahmed Z, Isaacs AM, Brelstaff JH, Borroni B, Warren JD, Troakes C, King A, Al-Saraj S, Newcombe J, Quinn N, Ostergaard K, Schrøder HD, Bojsen-Møller M, Braendgaard H, Fox NC, Rossor MN, Lees AJ, Holton JL, Revesz T
Brain : a journal of neurology 2011 Sep;134(Pt 9):2548-64

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Distribution and pattern of pathology in subjects with familial or sporadic late-onset cerebellar ataxia as assessed by p62/sequestosome immunohistochemistry.

Pikkarainen M, Hartikainen P, Soininen H, Alafuzoff I
Cerebellum (London, England) 2011 Dec;10(4):720-31

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Intracellular localization and splicing regulation of FUS/TLS are variably affected by amyotrophic lateral sclerosis-linked mutations.

Kino Y, Washizu C, Aquilanti E, Okuno M, Kurosawa M, Yamada M, Doi H, Nukina N
Nucleic acids research 2011 Apr;39(7):2781-98

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Frequency of ubiquitin and FUS-positive, TDP-43-negative frontotemporal lobar degeneration.

Seelaar H, Klijnsma KY, de Koning I, van der Lugt A, Chiu WZ, Azmani A, Rozemuller AJ, van Swieten JC
Journal of neurology 2010 May;257(5):747-53

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FUS-immunoreactive inclusions are a common feature in sporadic and non-SOD1 familial amyotrophic lateral sclerosis.

Deng HX, Zhai H, Bigio EH, Yan J, Fecto F, Ajroud K, Mishra M, Ajroud-Driss S, Heller S, Sufit R, Siddique N, Mugnaini E, Siddique T
Annals of neurology 2010 Jun;67(6):739-48

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FUS pathology defines the majority of tau- and TDP-43-negative frontotemporal lobar degeneration.

Urwin H, Josephs KA, Rohrer JD, Mackenzie IR, Neumann M, Authier A, Seelaar H, Van Swieten JC, Brown JM, Johannsen P, Nielsen JE, Holm IE, FReJA Consortium., Dickson DW, Rademakers R, Graff-Radford NR, Parisi JE, Petersen RC, Hatanpaa KJ, White CL 3rd, Weiner MF, Geser F, Van Deerlin VM, Trojanowski JQ, Miller BL, Seeley WW, van der Zee J, Kumar-Singh S, Engelborghs S, De Deyn PP, Van Broeckhoven C, Bigio EH, Deng HX, Halliday GM, Kril JJ, Munoz DG, Mann DM, Pickering-Brown SM, Doodeman V, Adamson G, Ghazi-Noori S, Fisher EM, Holton JL, Revesz T, Rossor MN, Collinge J, Mead S, Isaacs AM
Acta neuropathologica 2010 Jul;120(1):33-41

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A new subtype of frontotemporal lobar degeneration with FUS pathology.

Neumann M, Rademakers R, Roeber S, Baker M, Kretzschmar HA, Mackenzie IR
Brain : a journal of neurology 2009 Nov;132(Pt 11):2922-31

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FUS pathology in basophilic inclusion body disease.

Munoz DG, Neumann M, Kusaka H, Yokota O, Ishihara K, Terada S, Kuroda S, Mackenzie IR
Acta neuropathologica 2009 Nov;118(5):617-27

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Abundant FUS-immunoreactive pathology in neuronal intermediate filament inclusion disease.

Neumann M, Roeber S, Kretzschmar HA, Rademakers R, Baker M, Mackenzie IR
Acta neuropathologica 2009 Nov;118(5):605-16

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody [NBP1-89113] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody [NBP1-89113] - Analysis in human cell line HeLa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human cerebellum shows strong nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human colon shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human liver shows moderate nuclear positivity in bile duct cells while hepatocytes are negative.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - FFPE 5micron sections, Citrate pretreatment, 1:500 20m, Ultravision DAB detection. This image was submitted via customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues . Corresponding FUS RNA-seq data are presented for the same tissues.