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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-27531 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FUS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (99%), Rat (98%), Pig (99%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FUS using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FUS polyclonal antibody (Product # PA5-27531) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FUS using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FUS polyclonal antibody (Product # PA5-27531) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FUS was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUS Polyclonal Antibody (Product # PA5-27531) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FUS was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUS Polyclonal Antibody (Product # PA5-27531) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
