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- References [4]
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- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-562 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-562, RRID:AB_10002858
- Product name
- Rabbit Polyclonal FUS Antibody
- Antibody type
- Polyclonal
Submitted references ZFP36 RNA-binding proteins restrain T cell activation and anti-viral immunity.
TLS/FUS (translocated in liposarcoma/fused in sarcoma) regulates target gene transcription via single-stranded DNA response elements.
A comparative clinical, pathological, biochemical and genetic study of fused in sarcoma proteinopathies.
TLS inhibits RNA polymerase III transcription.
Moore MJ, Blachere NE, Fak JJ, Park CY, Sawicka K, Parveen S, Zucker-Scharff I, Moltedo B, Rudensky AY, Darnell RB
eLife 2018 May 31;7
eLife 2018 May 31;7
TLS/FUS (translocated in liposarcoma/fused in sarcoma) regulates target gene transcription via single-stranded DNA response elements.
Tan AY, Riley TR, Coady T, Bussemaker HJ, Manley JL
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 17;109(16):6030-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 17;109(16):6030-5
A comparative clinical, pathological, biochemical and genetic study of fused in sarcoma proteinopathies.
Lashley T, Rohrer JD, Bandopadhyay R, Fry C, Ahmed Z, Isaacs AM, Brelstaff JH, Borroni B, Warren JD, Troakes C, King A, Al-Saraj S, Newcombe J, Quinn N, Ostergaard K, Schrøder HD, Bojsen-Møller M, Braendgaard H, Fox NC, Rossor MN, Lees AJ, Holton JL, Revesz T
Brain : a journal of neurology 2011 Sep;134(Pt 9):2548-64
Brain : a journal of neurology 2011 Sep;134(Pt 9):2548-64
TLS inhibits RNA polymerase III transcription.
Tan AY, Manley JL
Molecular and cellular biology 2010 Jan;30(1):186-96
Molecular and cellular biology 2010 Jan;30(1):186-96
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and NB100-565 were used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Human skin squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody used at a dilution of 1:5,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
