Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-79787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PC1/3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PC1/3 in Lane 1: rat liver tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: A549 cell lysate, Lane 4: HeLa cell lysate, Lane 5: COLO320 cell lysate, Lane 6: PANC cell lysate. Sample was incubated with PC1/3 polyclonal antibody (Product # PA5-79787).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PC1/3 in Lane 1: rat liver tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: A549 cell lysate, Lane 4: HeLa cell lysate, Lane 5: COLO320 cell lysate, Lane 6: PANC cell lysate. Sample was incubated with PC1/3 polyclonal antibody (Product # PA5-79787).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PC1/3 on paraffin-embedded rat intestine tissue. Sample was incubated with PC1/3 polyclonal antibody (Product# PA5-79787).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PC1/3/PCSK1 in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-PC1/3/PCSK1 antibody (Product # PA5-79787) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin.The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
