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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29832 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCSK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116. Predicted reactivity: Mouse (99%), Rat (100%), Zebrafish (93%), Pig (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCSK1 Polyclonal Antibody (Product # PA5-29832). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. PCSK1 Polyclonal Antibody (Product # PA5-29832) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PC1/3 (Product # PA5-29832) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCSK1 Polyclonal Antibody detects PC1/3 protein at cytosol and secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PCSK1 Polyclonal Antibody (Product # PA5-29832) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
