NBP2-16542

antibody from Novus Biologicals

Targeting: FUBP1 FBP, FUBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16542

Provider

Novus Biologicals

Product name

Rabbit Polyclonal FUBP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUBP1 Antibody [NBP2-16542] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUBP1 Antibody [NBP2-16542] - Hela whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUBP1 Antibody [NBP2-16542] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of FUBP-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FUBP-1 by 2.5 ug of FUBP-1 antibody 7.5% SDS-PAGE The immunoprecipitated FUBP-1 protein was detected by FUBP-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FUBP1 Antibody [NBP2-16542] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FUBP1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - : Paraffin-embedded mouse hind brain. FUBP1 antibody dilution: 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP2-16542] - Paraffin-embedded mouse testis. FUBP1 antibody dilution: 1:1000.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: FUBP1 Antibody [NBP2-16542] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.