PA5-30291

antibody from Invitrogen Antibodies

Targeting: FUBP1 FBP, FUBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30291

Provider

Invitrogen Antibodies

Product name

FUBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HeLa nuclear extract, mouse brain, rat brain. Predicted reactivity: Mouse (99%), Rat (100%), Xenopus laevis (91%), Chicken (97%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FUBP1 using 30 µg of A) Jurkat (B) Raji (C) K562 (D) THP-1 (E) HL-60 and F) NCI-H929 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FUBP1 polyclonal antibody (Product # PA5-30291) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FUBP1 was performed by separating 30 µg of Hela whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUBP1 Polyclonal Antibody (Product # PA5-30291) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FUBP1 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FUBP1 Polyclonal Antibody (Product # PA5-30291) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FUBP1 in paraformaldehyde-fixed MCF-7 cells using a FUBP1 polyclonal antibody (Product # PA5-30291) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FUBP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 Polyclonal Antibody (Product # PA5-30291) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FUBP1 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 Polyclonal Antibody (Product # PA5-30291) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using FUBP1 (Product # PA5-30291) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.