A300-573A

antibody from Invitrogen Antibodies

Targeting: UBR5 DD5, EDD, EDD1, HYD, KIAA0896

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-573A

Provider

Invitrogen Antibodies

Product name

EDD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-EDD1 antibody (Product # A300-573A; lot A300-573A-6) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for IP at 3 µg per reaction. EDD1 was also immunoprecipitated by rabbit anti-EDD1 antibody A303-045A. For blotting immunoprecipitated EDD1, A300-573A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-EDD1 (Product # A300-573A; lot 6) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Product # A300-573A lot 6) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 5) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody (Product # A300-573A; lot A300-573A-6) used for IP at 6 µg per reaction. EDD1 was also immunoprecipitated by a previous lot of this antibody (lot A300-573A-5). For blotting immunoprecipitated EDD1 (Product # A300-573A) was used at 0.1 µg/mL. Chemiluminescence with an exposure time of 3 minutes.