AF6036

antibody from R&D Systems

Targeting: CPB2 CPU, PCPB, TAFI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6036

Provider

R&D Systems

Product name

Human Carboxypeptidase B2/CPB2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CPA1, rhCPB1 and recombinant mouse CPB1 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q96IY4


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human platelets. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase B2/CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxypeptidase B2/CPB2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Carboxypeptidase B2/CPB2 in Human Liver and Liver Cancer Tissue. Carboxypeptidase B2/CPB2 was detected in immersion fixed paraffin-embedded sections of normal human liver (left panel) and human liver cancer tissue (right panel) using Sheep Anti-Human Carboxypeptidase B2/ CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.