Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6036 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Carboxypeptidase B2/CPB2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Carboxypeptidase A1, A4, recombinant mouse (rm) Carboxypeptidase A1, A4, B1, and 5% cross-reactivity with rhCarboxypeptidase B1, B2, and E is observed under reducing conditions. No cross-reactivity with any of these proteins is observed under nonreducing conditions.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q96IY4
- Isotype
- IgG
- Antibody clone number
- 650801
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Carboxypeptidase B2/CPB2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Carboxypeptidase B2/CPB2 Monoclonal Antibody (Catalog # MAB6036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Carboxypeptidase B2/CPB2 at approximately 45 and 50 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.