700241

antibody from Invitrogen Antibodies

Targeting: PRKAB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

700241

Provider

Invitrogen Antibodies

Product name

Phospho-AMPK beta-1 (Ser182) Recombinant Rabbit Monoclonal Antibody (9H26L42)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, carp, chicken, equine, mouse, opossum, orangutan, platypus, rat, salmon, porcine, Xenopus and zebrafish based on sequence homology.

Antibody clone number

9H26L42

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-AMPK beta-1 pSer182 in Jurkat cell lysate using a Phospho-AMPK beta-1 pSer182 recombinant rabbit monoclonal antibody (Product # 700241) at a dilution of 2.5 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-AMPK beta-1 pSer182 in HeLa cells using a Phospho-AMPK beta-1 pSer182 recombinant rabbit monoclonal antibody (Product # 700241) at a dilution of 2.5 µg/mL in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-AMPK beta-1 pSer182 in formalin-fixed, paraffin-embedded human colon carcimona using a Phospho-AMPK beta-1 pSer182 monoclonal antibody (Product # 700241) at a dilution of 5 µg/mL. Tissues were pretreated with EDTA and staining was visualized using DAB. Images were taken at a magnification of 40x. Results show nuclear staining in tumor cells.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Phospho-AMPK beta-1 pSer182 in Jurkat cells using a Phospho-AMPK beta-1 pSer182 recombinant rabbit monoclonal antibody (Product # 700241) at a dilution of 0.5ug. Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to a control without primary antibody (gray). Pre-incubation with the phosphopeptide decreased the signal (red) whereas incubation with the non-phosphopeptide did not (blue).