H00005564-M01

antibody from Abnova Corporation

Targeting: PRKAB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005564-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005564-M01, RRID:AB_425611

Product name

PRKAB1 monoclonal antibody (M01), clone 3H12-1A10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PRKAB1.

Antigen sequence

MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPK
ILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVN
DKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPL
TRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEP
IVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSD
VSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHL
LQVILNKDTGISCDPALLPEPNHVMLNHLYALSIK
DGVMVLSATHRYKKKYVTTLLYKPI


Isotype

IgG

Antibody clone number

3H12-1A10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PRKAB1 expression in transfected 293T cell line by PRKAB1 monoclonal antibody (M01), clone 3H12-1A10.Lane 1: PRKAB1 transfected lysate(31.05 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PRKAB1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol