Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47411 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK beta-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-human/mouse/Rat AMPKβ1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47411) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. For additional reference, recombinant human AMPKβ1 and AMPKβ2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK beta-1 in HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. Samples were incubated in AMPK beta-1 polyclonal antibody (Product # PA5-47411) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, recombinant human AMPKβ1 and AMPKβ2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions.