PA5-47411

antibody from Invitrogen Antibodies

Targeting: PRKAB1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-47411

Provider

Invitrogen Antibodies

Product name

AMPK beta-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-human/mouse/Rat AMPKβ1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47411) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. For additional reference, recombinant human AMPKβ1 and AMPKβ2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK beta-1 in HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. Samples were incubated in AMPK beta-1 polyclonal antibody (Product # PA5-47411) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. For additional reference, recombinant human AMPKβ1 and AMPKβ2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions.