LS-C761166

antibody from LSBio

Targeting: PRKAB1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C761166

Provider

LSBio

Product name

PRKAB1 / AMPK Beta 1 Antibody LS-C761166

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Storage

Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of AMPK beta 1 expression in Hela (A), HepG2 (B), K562 (C), PC3 (D), MCF7 (E), mouse spleen (F) whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of AMPK beta 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.