- PA5-35341 - Invitrogen Antibodies SCAP antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SCAP in Hela cells. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and probed with a SCAP polyclonal antibody (Product # PA5-35341) at a dilution of 1:100 for 2 hours at room temperature, followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of SCAP in HeLa cells. Samples were incubated with SCAP polyclonal antibody (Product # PA5-35341) using a dilution of 1:100 for 2 h at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:1,000 for 1hr. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SCAP in formalin-fixed and paraffin-embedded human colon carcinoma. Samples were incubated with SCAP polyclonal antibody (Product # PA5-35341) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of SCAP in K562 cells (bottom) compared to a negative control (top) using a SCAP polyclonal antibody (Product # PA5-35341) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of (overlay histogram) of SCAP in K562 cells (green line). Samples were incubated with SCAP polyclonal antibody (Product # PA5-35341) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of (overlay histogram) of SCAP in K562 cells (green line). Samples were incubated with SCAP polyclonal antibody (Product # PA5-35341) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

PA5-35341