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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-35341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SCAP in Hela cells. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and probed with a SCAP polyclonal antibody (Product # PA5-35341) at a dilution of 1:100 for 2 hours at room temperature, followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SCAP in K562 cells (bottom) compared to a negative control (top) using a SCAP polyclonal antibody (Product # PA5-35341) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
