Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP7050c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7050c, RRID:AB_2168059
- Product name
- PRKAG3 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Expression of 5'-AMP-activated protein kinase with starvation in murine thymocytes.
Hypoxia induces expression and activation of AMPK in rat dental pulp cells.
Okoshi R, Ohta K, Ishikawa A, Sato Y, Kizaki H
The Bulletin of Tokyo Dental College 2011;52(1):21-9
The Bulletin of Tokyo Dental College 2011;52(1):21-9
Hypoxia induces expression and activation of AMPK in rat dental pulp cells.
Fukuyama Y, Ohta K, Okoshi R, Suehara M, Kizaki H, Nakagawa K
Journal of dental research 2007 Sep;86(9):903-7
Journal of dental research 2007 Sep;86(9):903-7
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PRKAG3 Pab (Cat. #AP7050c) in mouse brain tissue lysate. PRKAG3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100