BS-2930R

antibody from Invitrogen Antibodies

Targeting: FZD8

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-2930R

Provider

Invitrogen Antibodies

Product name

Frizzled 5/8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L1 rat lung lysates, L2 rat brain lysates probed (bs-2930R) Anti-Frizzled-5 Polyclonal, Unconjugated at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band and observed band size: 62 kD.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K562 cell lysates probed with Rabbit Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: Cerebrum lysates probed with Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Frizzled 5/8 Polyclonal Antibody, Unconjugated (bs-2930R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.