Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28081 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FZD8 Polyclonal Antibody (Product # PA5-28081). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. FZD8 Polyclonal Antibody (Product # PA5-28081) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FZD8 Polyclonal Antibody (Product # PA5-28081). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FZD8 Polyclonal Antibody (Product # PA5-28081) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Frizzled 8 (Product # PA5-28081) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.