Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-48298 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer.
Lin LL, Kost ER, Lin CL, Valente P, Wang CM, Kolonin MG, Daquinag AC, Tan X, Lucio N, Hung CN, Wang CP, Kirma NB, Huang TH
Cell reports 2020 Oct 13;33(2):108253
Cell reports 2020 Oct 13;33(2):108253
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAS1 in NCI-H460 cells. Cells were stained with a GAS1 Antibody (N-term) (Product # PA5-48298) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GAS1 in NCI-H460 cells. Samples were incubated in GAS1 polyclonal antibody (Product # PA5-48298) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GAS1 in formalin fixed and paraffin embedded mouse heart tissue. Samples were incubated with GAS1 polyclonal antibody (Product # PA5-48298) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of GAS1 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a GAS1 Antibody (N-term) (Product # PA5-48298). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GAS1 in NCI-H460 cells (right histogram). Samples were incubated with GAS1 polyclonal antibody (Product # PA5-48298) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
