702555

antibody from Invitrogen Antibodies

Targeting: FOXN3 C14orf116, CHES1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

702555

Provider

Invitrogen Antibodies

Product name

FOXN3 Recombinant Rabbit Monoclonal Antibody (1H2L5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Horse, Cat

Antibody clone number

1H2L5

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Nuclear enriched cell extracts (30 µg lysate) of Jurkat (Lane 1), Hep G2 (Lane 2), Karpas 299 (Lane 3), HT-29 (Lane 4), THP-1 (Lane 5), SH-SY5Y (Lane 6), U-2 OS (Lane 7) and PC-3 (Lane 8). The blots were probed with Anti-FOXN3 Recombinant Rabbit Monoclonal Antibody (Product # 702555, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 54 kDa band corresponding to FOXN3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).