358408
antibody from BioLegend
Targeting: TNFRSF1B
CD120b, p75, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- 358408 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#358408, RRID:AB_2563224
- Product name
- Ultra-LEAF™ Purified anti-human CD120b
- Antibody type
- Monoclonal
- Antigen
- Partial CD120b recombinant protein expressed in 293E cells (22-211aa).
- Description
- CD120b, also known as TNFRII or TNFRSF1B, belongs to the TNF receptor superfamily and can interact with ligands TNF-α and TNF-β (also known as LT-α). It is a 75 kD type I transmembrane protein expressed on lymphocytes, monocytes, and granulocytes. When present in a heterocomplex with CD120a, it induces the recruitment of anti-apoptotic molecules, c-IAP1 and c-IAP2 that possess E3 ubiquitin ligase activity. Mutations in CD120b are associated with TNF-associated periodic syndrome (TRAPS) or periodic fever syndrome.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 3G7A02
- Vial size
- 500 µg
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- TNF RII/TNFRSF1B Inhibition of TNFâalpha-induced Cytotoxicity and Neutralization by Human CD120b Antibody. Recombinant Human sTNF RII (Catalog # 591602) inhibits Recombinant Human TNFâalpha (Catalog # 570102) induced cytotoxicity in the Lâ929 mouse fibroblast cell line in a dose-dependent manner (black line). Inhibition of Recombinant Human TNFâalpha (0.25 ng/mL) activity elicited by Recombinant Human sTNF RII (0.4 µg/mL) is neutralized by increasing concentrations of Human CD120b Monoclonal Antibody (Red line) in the presence of the metabolic inhibitor actinomycin D (1 µg/ml). The ED50 is 0.5-1.5 µg/ml.
- Conjugate
- Unconjugated