A21995

antibody from Invitrogen Antibodies

Targeting: OGDH E1k, KGD1, OGDC-E1, OGDH2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A21995

Provider

Invitrogen Antibodies

Product name

Anti-OGDH Monoclonal Antibody (9F4BD5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

9F4BD5

Vial size

100 µg

Concentration

1 mg/ml

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody at 1 µg/mL overnight at 4°C. Secondary antibody: (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.