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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX124178 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX124178, RRID:AB_11168478
- Product name
- E-Cadherin antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κB-dependent MMP-9 and epithelial-mesenchymal transition expressions.
Ling HH, Kuo CC, Lin BX, Huang YH, Lin CW
Experimental cell research 2017 Jan 1;350(1):218-225
Experimental cell research 2017 Jan 1;350(1):218-225
Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κB-dependent MMP-9 and epithelial-mesenchymal transition expressions.
Lee WT, Lee TH, Cheng CH, Chen KC, Chen YC, Lin CW
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Apr;78:33-41
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Apr;78:33-41
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T 5% SDS PAGE GTX124178 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- E-Cadherin antibody [N3C2], Internal detects E-Cadherin protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [N3C2], Internal (GTX124178) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using E-cadherin(GTX124178) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 £gg of E-Cadherin antibody [N3C2], Internal (GTX124178).Western blot analysis was performed using E-Cadherin antibody [N3C2], Internal (GTX124178).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
