Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [54]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [22]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-5700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E-cadherin Monoclonal Antibody (SHE78-7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Source: Monoclonal antibody was obtained by fusing the P3U1 myeloma cell line with spleen cells of BALB/c mouse after immunization with soluble E-cadherin from human placenta.
- Antibody clone number
- SHE78-7
- Concentration
- 2.0 mg/mL
Submitted references Extracellular Vesicles from M1-Polarized Macrophages Combined with Hyaluronic Acid and a β-Blocker Potentiate Doxorubicin's Antitumor Activity by Downregulating Tumor-Associated Macrophages in Breast Cancer.
CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion.
Oncogenic PI3K/AKT promotes the step-wise evolution of combination BRAF/MEK inhibitor resistance in melanoma.
Statins attenuate outgrowth of breast cancer metastases.
Post-translational modification of the interferon-gamma receptor alters its stability and signaling.
Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier.
Liver protects metastatic prostate cancer from induced death by activating E-cadherin signaling.
Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA.
RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.
The role of multicellular aggregation in the survival of ErbB2-positive breast cancer cells during extracellular matrix detachment.
Oncogenic B-RAF(V600E) signaling induces the T-Box3 transcriptional repressor to repress E-cadherin and enhance melanoma cell invasion.
A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation.
Silk film topography directs collective epithelial cell migration.
Loss of E-cadherin in multidrug resistant breast cancer cell line MCF-7/Adr: possible implication in the enhanced invasive ability.
Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor.
Using cadherin expression to assess spontaneous differentiation of embryonic stem cells.
α-Catenin contributes to the strength of E-cadherin-p120 interactions.
Cadherin exits the junction by switching its adhesive bond.
The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy.
Abrogation of E-cadherin-mediated cellular aggregation allows proliferation of pluripotent mouse embryonic stem cells in shake flask bioreactors.
Myosin II isoforms identify distinct functional modules that support integrity of the epithelial zonula adherens.
Tumor suppressor function of BCSC-1 in nasopharyngeal carcinoma.
Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells.
Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.
Anti-oestrogens but not oestrogen deprivation promote cellular invasion in intercellular adhesion-deficient breast cancer cells.
p120-catenin is a key component of the cadherin-gamma-secretase supercomplex.
Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization.
Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies.
Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation.
Stable and unstable cadherin dimers: mechanisms of formation and roles in cell adhesion.
Cytotrophoblasts infected with a pathogenic human cytomegalovirus strain dysregulate cell-matrix and cell-cell adhesion molecules: a quantitative analysis.
Notch1 signaling promotes primary melanoma progression by activating mitogen-activated protein kinase/phosphatidylinositol 3-kinase-Akt pathways and up-regulating N-cadherin expression.
Role of notch-1 and E-cadherin in the differential response to calcium in culturing normal versus malignant prostate cells.
Cyclooxygenase-2 overexpression in MCF-10F human breast epithelial cells inhibits proliferation, apoptosis and differentiation, and causes partial transformation.
Reduced E-cadherin expression contributes to the loss of p27kip1-mediated mechanism of contact inhibition in thyroid anaplastic carcinomas.
Osteopontin expression in ductal adenocarcinomas and undifferentiated carcinomas of the pancreas.
EGFR signaling to p120-catenin through phosphorylation at Y228.
The relative contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha transcriptional activity depends upon the differentiation stage of the cell.
Reversal of melanocytic malignancy by keratinocytes is an E-cadherin-mediated process overriding beta-catenin signaling.
A core function for p120-catenin in cadherin turnover.
N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells.
E-cadherin expression in melanoma cells restores keratinocyte-mediated growth control and down-regulates expression of invasion-related adhesion receptors.
Molecular basis for leukocyte integrin alpha(E)beta(7) adhesion to epithelial (E)-cadherin.
High levels of E-/P-cadherin: correlation with decreased apical polarity of Na/K ATPase in bovine RPE cells in situ.
p21WAF1 regulates anchorage-independent growth of HCT116 colon carcinoma cells via E-cadherin expression.
Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution.
Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution.
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway.
E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway.
E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells.
E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells.
E-Cadherin-dependent growth suppression is mediated by the cyclin-dependent kinase inhibitor p27(KIP1).
Jorquera-Cordero C, Lara P, Cruz LJ, Schomann T, van Hofslot A, de Carvalho TG, Guedes PMDM, Creemers L, Koning RI, Chan AB, de Araujo Junior RF
Pharmaceutics 2022 May 17;14(5)
Pharmaceutics 2022 May 17;14(5)
CD103 (αE Integrin) Undergoes Endosomal Trafficking in Human Dendritic Cells, but Does Not Mediate Epithelial Adhesion.
Swain S, Roe MM, Sebrell TA, Sidar B, Dankoff J, VanAusdol R, Smythies LE, Smith PD, Bimczok D
Frontiers in immunology 2018;9:2989
Frontiers in immunology 2018;9:2989
Oncogenic PI3K/AKT promotes the step-wise evolution of combination BRAF/MEK inhibitor resistance in melanoma.
Irvine M, Stewart A, Pedersen B, Boyd S, Kefford R, Rizos H
Oncogenesis 2018 Sep 20;7(9):72
Oncogenesis 2018 Sep 20;7(9):72
Statins attenuate outgrowth of breast cancer metastases.
Beckwitt CH, Clark AM, Ma B, Whaley D, Oltvai ZN, Wells A
British journal of cancer 2018 Oct;119(9):1094-1105
British journal of cancer 2018 Oct;119(9):1094-1105
Post-translational modification of the interferon-gamma receptor alters its stability and signaling.
Londino JD, Gulick DL, Lear TB, Suber TL, Weathington NM, Masa LS, Chen BB, Mallampalli RK
The Biochemical journal 2017 Oct 10;474(20):3543-3557
The Biochemical journal 2017 Oct 10;474(20):3543-3557
Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier.
Goyer M, Loiselet A, Bon F, L'Ollivier C, Laue M, Holland G, Bonnin A, Dalle F
PloS one 2016;11(3):e0149159
PloS one 2016;11(3):e0149159
Liver protects metastatic prostate cancer from induced death by activating E-cadherin signaling.
Ma B, Wheeler SE, Clark AM, Whaley DL, Yang M, Wells A
Hepatology (Baltimore, Md.) 2016 Nov;64(5):1725-1742
Hepatology (Baltimore, Md.) 2016 Nov;64(5):1725-1742
Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA.
Schmidt TP, Perna AM, Fugmann T, Böhm M, Jan Hiss, Haller S, Götz C, Tegtmeyer N, Hoy B, Rau TT, Neri D, Backert S, Schneider G, Wessler S
Scientific reports 2016 Mar 17;6:23264
Scientific reports 2016 Mar 17;6:23264
RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.
Kajiho H, Kajiho Y, Frittoli E, Confalonieri S, Bertalot G, Viale G, Di Fiore PP, Oldani A, Garre M, Beznoussenko GV, Palamidessi A, Vecchi M, Chavrier P, Perez F, Scita G
EMBO reports 2016 Jul;17(7):1061-80
EMBO reports 2016 Jul;17(7):1061-80
The role of multicellular aggregation in the survival of ErbB2-positive breast cancer cells during extracellular matrix detachment.
Rayavarapu RR, Heiden B, Pagani N, Shaw MM, Shuff S, Zhang S, Schafer ZT
The Journal of biological chemistry 2015 Apr 3;290(14):8722-33
The Journal of biological chemistry 2015 Apr 3;290(14):8722-33
Oncogenic B-RAF(V600E) signaling induces the T-Box3 transcriptional repressor to repress E-cadherin and enhance melanoma cell invasion.
Boyd SC, Mijatov B, Pupo GM, Tran SL, Gowrishankar K, Shaw HM, Goding CR, Scolyer RA, Mann GJ, Kefford RF, Rizos H, Becker TM
The Journal of investigative dermatology 2013 May;133(5):1269-77
The Journal of investigative dermatology 2013 May;133(5):1269-77
A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation.
Weiswald LB, Richon S, Massonnet G, Guinebretière JM, Vacher S, Laurendeau I, Cottu P, Marangoni E, Nemati F, Validire P, Bellet D, Bièche I, Dangles-Marie V
British journal of cancer 2013 Apr 30;108(8):1720-31
British journal of cancer 2013 Apr 30;108(8):1720-31
Silk film topography directs collective epithelial cell migration.
Lawrence BD, Pan Z, Rosenblatt MI
PloS one 2012;7(11):e50190
PloS one 2012;7(11):e50190
Loss of E-cadherin in multidrug resistant breast cancer cell line MCF-7/Adr: possible implication in the enhanced invasive ability.
Lu L, Zhou D, Jiang X, Song K, Li K, Ding W
European review for medical and pharmacological sciences 2012 Sep;16(9):1271-9
European review for medical and pharmacological sciences 2012 Sep;16(9):1271-9
Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor.
Garnier D, Magnus N, Lee TH, Bentley V, Meehan B, Milsom C, Montermini L, Kislinger T, Rak J
The Journal of biological chemistry 2012 Dec 21;287(52):43565-72
The Journal of biological chemistry 2012 Dec 21;287(52):43565-72
Using cadherin expression to assess spontaneous differentiation of embryonic stem cells.
Spencer H, Keramari M, Ward CM
Methods in molecular biology (Clifton, N.J.) 2011;690:81-94
Methods in molecular biology (Clifton, N.J.) 2011;690:81-94
α-Catenin contributes to the strength of E-cadherin-p120 interactions.
Troyanovsky RB, Klingelhöfer J, Troyanovsky SM
Molecular biology of the cell 2011 Nov;22(22):4247-55
Molecular biology of the cell 2011 Nov;22(22):4247-55
Cadherin exits the junction by switching its adhesive bond.
Hong S, Troyanovsky RB, Troyanovsky SM
The Journal of cell biology 2011 Mar 21;192(6):1073-83
The Journal of cell biology 2011 Mar 21;192(6):1073-83
The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy.
Xiang X, Phung Y, Feng M, Nagashima K, Zhang J, Broaddus VC, Hassan R, Fitzgerald D, Ho M
PloS one 2011 Jan 31;6(1):e14640
PloS one 2011 Jan 31;6(1):e14640
Abrogation of E-cadherin-mediated cellular aggregation allows proliferation of pluripotent mouse embryonic stem cells in shake flask bioreactors.
Mohamet L, Lea ML, Ward CM
PloS one 2010 Sep 23;5(9):e12921
PloS one 2010 Sep 23;5(9):e12921
Myosin II isoforms identify distinct functional modules that support integrity of the epithelial zonula adherens.
Smutny M, Cox HL, Leerberg JM, Kovacs EM, Conti MA, Ferguson C, Hamilton NA, Parton RG, Adelstein RS, Yap AS
Nature cell biology 2010 Jul;12(7):696-702
Nature cell biology 2010 Jul;12(7):696-702
Tumor suppressor function of BCSC-1 in nasopharyngeal carcinoma.
Zhou YQ, Chen SL, Ju JY, Shen L, Liu Y, Zhen S, Lv N, He ZG, Zhu LP
Cancer science 2009 Oct;100(10):1817-22
Cancer science 2009 Oct;100(10):1817-22
Interaction of E-cadherin and PTEN regulates morphogenesis and growth arrest in human mammary epithelial cells.
Fournier MV, Fata JE, Martin KJ, Yaswen P, Bissell MJ
Cancer research 2009 May 15;69(10):4545-52
Cancer research 2009 May 15;69(10):4545-52
Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.
den Elzen N, Buttery CV, Maddugoda MP, Ren G, Yap AS
Molecular biology of the cell 2009 Aug;20(16):3740-50
Molecular biology of the cell 2009 Aug;20(16):3740-50
Anti-oestrogens but not oestrogen deprivation promote cellular invasion in intercellular adhesion-deficient breast cancer cells.
Borley AC, Hiscox S, Gee J, Smith C, Shaw V, Barrett-Lee P, Nicholson RI
Breast cancer research : BCR 2008;10(6):R103
Breast cancer research : BCR 2008;10(6):R103
p120-catenin is a key component of the cadherin-gamma-secretase supercomplex.
Kiss A, Troyanovsky RB, Troyanovsky SM
Molecular biology of the cell 2008 Oct;19(10):4042-50
Molecular biology of the cell 2008 Oct;19(10):4042-50
Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization.
Marín-Briggiler CI, Veiga MF, Matos ML, Echeverría MF, Furlong LI, Vazquez-Levin MH
Molecular human reproduction 2008 Oct;14(10):561-71
Molecular human reproduction 2008 Oct;14(10):561-71
Cadherin switching dictates the biology of transitional cell carcinoma of the bladder: ex vivo and in vitro studies.
Bryan RT, Atherfold PA, Yeo Y, Jones LJ, Harrison RF, Wallace DM, Jankowski JA
The Journal of pathology 2008 Jun;215(2):184-94
The Journal of pathology 2008 Jun;215(2):184-94
Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation.
Haga T, Uchide N, Tugizov S, Palefsky JM
Apoptosis : an international journal on programmed cell death 2008 Jan;13(1):97-108
Apoptosis : an international journal on programmed cell death 2008 Jan;13(1):97-108
Stable and unstable cadherin dimers: mechanisms of formation and roles in cell adhesion.
Troyanovsky RB, Laur O, Troyanovsky SM
Molecular biology of the cell 2007 Nov;18(11):4343-52
Molecular biology of the cell 2007 Nov;18(11):4343-52
Cytotrophoblasts infected with a pathogenic human cytomegalovirus strain dysregulate cell-matrix and cell-cell adhesion molecules: a quantitative analysis.
Tabata T, McDonagh S, Kawakatsu H, Pereira L
Placenta 2007 May-Jun;28(5-6):527-37
Placenta 2007 May-Jun;28(5-6):527-37
Notch1 signaling promotes primary melanoma progression by activating mitogen-activated protein kinase/phosphatidylinositol 3-kinase-Akt pathways and up-regulating N-cadherin expression.
Liu ZJ, Xiao M, Balint K, Smalley KS, Brafford P, Qiu R, Pinnix CC, Li X, Herlyn M
Cancer research 2006 Apr 15;66(8):4182-90
Cancer research 2006 Apr 15;66(8):4182-90
Role of notch-1 and E-cadherin in the differential response to calcium in culturing normal versus malignant prostate cells.
Dalrymple S, Antony L, Xu Y, Uzgare AR, Arnold JT, Savaugeot J, Sokoll LJ, De Marzo AM, Isaacs JT
Cancer research 2005 Oct 15;65(20):9269-79
Cancer research 2005 Oct 15;65(20):9269-79
Cyclooxygenase-2 overexpression in MCF-10F human breast epithelial cells inhibits proliferation, apoptosis and differentiation, and causes partial transformation.
Lu S, Yu G, Zhu Y, Archer MC
International journal of cancer 2005 Oct 10;116(6):847-52
International journal of cancer 2005 Oct 10;116(6):847-52
Reduced E-cadherin expression contributes to the loss of p27kip1-mediated mechanism of contact inhibition in thyroid anaplastic carcinomas.
Motti ML, Califano D, Baldassarre G, Celetti A, Merolla F, Forzati F, Napolitano M, Tavernise B, Fusco A, Viglietto G
Carcinogenesis 2005 Jun;26(6):1021-34
Carcinogenesis 2005 Jun;26(6):1021-34
Osteopontin expression in ductal adenocarcinomas and undifferentiated carcinomas of the pancreas.
Sedivy R, Peters K, Klöppel G
Virchows Archiv : an international journal of pathology 2005 Jan;446(1):41-5
Virchows Archiv : an international journal of pathology 2005 Jan;446(1):41-5
EGFR signaling to p120-catenin through phosphorylation at Y228.
Mariner DJ, Davis MA, Reynolds AB
Journal of cell science 2004 Mar 15;117(Pt 8):1339-50
Journal of cell science 2004 Mar 15;117(Pt 8):1339-50
The relative contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha transcriptional activity depends upon the differentiation stage of the cell.
Mérot Y, Métivier R, Penot G, Manu D, Saligaut C, Gannon F, Pakdel F, Kah O, Flouriot G
The Journal of biological chemistry 2004 Jun 18;279(25):26184-91
The Journal of biological chemistry 2004 Jun 18;279(25):26184-91
Reversal of melanocytic malignancy by keratinocytes is an E-cadherin-mediated process overriding beta-catenin signaling.
Li G, Fukunaga M, Herlyn M
Experimental cell research 2004 Jul 1;297(1):142-51
Experimental cell research 2004 Jul 1;297(1):142-51
A core function for p120-catenin in cadherin turnover.
Davis MA, Ireton RC, Reynolds AB
The Journal of cell biology 2003 Nov 10;163(3):525-34
The Journal of cell biology 2003 Nov 10;163(3):525-34
N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells.
Li G, Satyamoorthy K, Herlyn M
Cancer research 2001 May 1;61(9):3819-25
Cancer research 2001 May 1;61(9):3819-25
E-cadherin expression in melanoma cells restores keratinocyte-mediated growth control and down-regulates expression of invasion-related adhesion receptors.
Hsu MY, Meier FE, Nesbit M, Hsu JY, Van Belle P, Elder DE, Herlyn M
The American journal of pathology 2000 May;156(5):1515-25
The American journal of pathology 2000 May;156(5):1515-25
Molecular basis for leukocyte integrin alpha(E)beta(7) adhesion to epithelial (E)-cadherin.
Taraszka KS, Higgins JM, Tan K, Mandelbrot DA, Wang JH, Brenner MB
The Journal of experimental medicine 2000 May 1;191(9):1555-67
The Journal of experimental medicine 2000 May 1;191(9):1555-67
High levels of E-/P-cadherin: correlation with decreased apical polarity of Na/K ATPase in bovine RPE cells in situ.
Burke JM, Cao F, Irving PE
Investigative ophthalmology & visual science 2000 Jun;41(7):1945-52
Investigative ophthalmology & visual science 2000 Jun;41(7):1945-52
p21WAF1 regulates anchorage-independent growth of HCT116 colon carcinoma cells via E-cadherin expression.
Mueller S, Cadenas E, Schönthal AH
Cancer research 2000 Jan 1;60(1):156-63
Cancer research 2000 Jan 1;60(1):156-63
Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution.
Vallorosi CJ, Day KC, Zhao X, Rashid MG, Rubin MA, Johnson KR, Wheelock MJ, Day ML
The Journal of biological chemistry 2000 Feb 4;275(5):3328-34
The Journal of biological chemistry 2000 Feb 4;275(5):3328-34
Truncation of the beta-catenin binding domain of E-cadherin precedes epithelial apoptosis during prostate and mammary involution.
Vallorosi CJ, Day KC, Zhao X, Rashid MG, Rubin MA, Johnson KR, Wheelock MJ, Day ML
The Journal of biological chemistry 2000 Feb 4;275(5):3328-34
The Journal of biological chemistry 2000 Feb 4;275(5):3328-34
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Hines MD, Jin HC, Wheelock MJ, Jensen PJ
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
Hines MD, Jin HC, Wheelock MJ, Jensen PJ
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
Journal of cell science 1999 Dec;112 ( Pt 24):4569-79
E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway.
Day ML, Zhao X, Vallorosi CJ, Putzi M, Powell CT, Lin C, Day KC
The Journal of biological chemistry 1999 Apr 2;274(14):9656-64
The Journal of biological chemistry 1999 Apr 2;274(14):9656-64
E-cadherin mediates aggregation-dependent survival of prostate and mammary epithelial cells through the retinoblastoma cell cycle control pathway.
Day ML, Zhao X, Vallorosi CJ, Putzi M, Powell CT, Lin C, Day KC
The Journal of biological chemistry 1999 Apr 2;274(14):9656-64
The Journal of biological chemistry 1999 Apr 2;274(14):9656-64
E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells.
Kantak SS, Kramer RH
The Journal of biological chemistry 1998 Jul 3;273(27):16953-61
The Journal of biological chemistry 1998 Jul 3;273(27):16953-61
E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells.
Kantak SS, Kramer RH
The Journal of biological chemistry 1998 Jul 3;273(27):16953-61
The Journal of biological chemistry 1998 Jul 3;273(27):16953-61
E-Cadherin-dependent growth suppression is mediated by the cyclin-dependent kinase inhibitor p27(KIP1).
St Croix B, Sheehan C, Rak JW, Flørenes VA, Slingerland JM, Kerbel RS
The Journal of cell biology 1998 Jul 27;142(2):557-71
The Journal of cell biology 1998 Jul 27;142(2):557-71
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-E-cadherin Monoclonal Antibody (SHE78-7)(Product # 13-5700) and a 110kDa band corresponding to E-cadherin was observed across in all tested cell lines and tissues, except MDA-MB-231, SW480 and BJ. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), MDA-MB-231 (Lane 3), SW480 (Lane 4), Caco-2 (Lane 5), BJ (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).The bands at ~80kDa and 60kDa corresponds to proteolytic cleavage products of E-cadherin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Sensitizing spheroids to immunotoxin therapy in vitro . A . Silencing E-Cadherin expression by siRNA. siE-Cad, E-Cadherin siRNA. 2D: monolayers; 3D: spheroids. B. Silencing E-Cadherin enhanced the anti-tumor activity of SS1P. Cell growth was assessed by incubation with WST-8 with values normalized (%) relative to the growth of the non-silencing siRNA (* p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Expression of cell junction and Bcl-2 signaling proteins in NCI-H226 monolayers and spheroids. A . Protein expression of E-Cadherin, ZO-1, Connexin-32, Mcl-1, Bcl-xL, Bid, Bak, Bax, and beta-actin examined by Western blot. B . Molecular mechanisms underlying drug resistance in tumor spheroids.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 6 Modulation of RT4 growth on collagen after 5 days. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C) RT4 growth in the presence of anti-P-cadherin function-blocking antibody. (A-C) All show RT4 forming tight rounded multicellular colonies without any noticeable morphological changes. (D) RT4 growth in the presence of anti-E-cadherin function-blocking antibody, with the cells clustering together but seemingly unable to form tight rounded multicellular colonies
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Modulation of RT4 growth on plastic after 24 h. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C, D) RT4 growth in the presence of anti-P-cadherin function blocking antibody. (E, F) RT4 growth in the presence of anti-E-cadherin function-blocking antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Atorvastatin suppresses mesenchymal breast cancer cells. MCF-7 RFP, MDA-MB-231 RFP, and MDA-MB-231 RFP/Ecad were grown in the presence of atorvastatin for 72 h and total cell number was determined by crystal violet staining and normalized to the control ( a ). IC 50 values for each cell line are reported ( a ). MCF-7 RFP, MDA-MB-231 RFP, and MDA-MB-231 RFP/Ecad were seeded either at 7.5 x 10 4 or 20 x 10 4 cells per well and probed for their total cell levels of E-cadherin and vimentin ( b ). The expression of E-cadherin ( c - e ) and vimentin ( f - h ) in MCF-7 RFP ( c , f ), MDA-MB-231 RFP ( d , g ), and MDA-MB-231 RFP/Ecad ( e , h ) was probed by immunofluorescent imaging. The data are representative of three independent experiments. Scalebar = 50 um
