Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAF748 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse E-Cadherin Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse E-Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) E-Cadherin, rhN-Cadherin, recombinant mouse (rm) P-Cadherin and rmVE-Cadherin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P09803
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Combined Mutation of Apc, Kras, and Tgfbr2 Effectively Drives Metastasis of Intestinal Cancer.
Quantifying cell-generated mechanical forces within living embryonic tissues.
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Sakai E, Nakayama M, Oshima H, Kouyama Y, Niida A, Fujii S, Ochiai A, Nakayama KI, Mimori K, Suzuki Y, Hong CP, Ock CY, Kim SJ, Oshima M
Cancer research 2018 Mar 1;78(5):1334-1346
Cancer research 2018 Mar 1;78(5):1334-1346
Quantifying cell-generated mechanical forces within living embryonic tissues.
Campàs O, Mammoto T, Hasso S, Sperling RA, O'Connell D, Bischof AG, Maas R, Weitz DA, Mahadevan L, Ingber DE
Nature methods 2014 Feb;11(2):183-9
Nature methods 2014 Feb;11(2):183-9
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
Cell stem cell 2008 Jul 3;3(1):55-68
Cell stem cell 2008 Jul 3;3(1):55-68
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- E-Cadherin in A431 Human Cell Line. E-Cadherin was detected in immersion fixed A431 human skin epidermal cell line using Goat Anti-Mouse E-Cadherin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.