Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF748 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse E-Cadherin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse E-Cadherin in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P09803
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Loss of BCL9/9l suppresses Wnt driven tumourigenesis in models that recapitulate human cancer.
Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication.
Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model.
Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination.
Combined Mutation of Apc, Kras, and Tgfbr2 Effectively Drives Metastasis of Intestinal Cancer.
Multicolor quantitative confocal imaging cytometry.
NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes.
Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner.
Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic α Cell Hyperplasia in Mice.
Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection.
A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease.
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
E-cadherin can limit the transforming properties of activating β-catenin mutations.
AKT3 regulates ErbB2, ErbB3 and estrogen receptor α expression and contributes to endocrine therapy resistance of ErbB2(+) breast tumor cells from Balb-neuT mice.
Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion.
Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells.
Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules.
EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
MMP20 modulates cadherin expression in ameloblasts as enamel develops.
A critical role for TLR4 induction of autophagy in the regulation of enterocyte migration and the pathogenesis of necrotizing enterocolitis.
N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs.
Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
Noninvasive assessment of antenatal hydronephrosis in mice reveals a critical role for Robo2 in maintaining anti-reflux mechanism.
Effect of inhibition of vascular endothelial growth factor signaling on distribution of extravasated antibodies in tumors.
FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
Multifunctionality of PAI-1 in fibrogenesis: evidence from obstructive nephropathy in PAI-1-overexpressing mice.
Gay DM, Ridgway RA, Müller M, Hodder MC, Hedley A, Clark W, Leach JD, Jackstadt R, Nixon C, Huels DJ, Campbell AD, Bird TG, Sansom OJ
Nature communications 2019 Feb 13;10(1):723
Nature communications 2019 Feb 13;10(1):723
Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication.
Wang J, Batourina E, Schneider K, Souza S, Swayne T, Liu C, George CD, Tate T, Dan H, Wiessner G, Zhuravlev Y, Canman JC, Mysorekar IU, Mendelsohn CL
Cell reports 2018 Oct 9;25(2):464-477.e4
Cell reports 2018 Oct 9;25(2):464-477.e4
Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model.
Flemming S, Luissint AC, Nusrat A, Parkos CA
JCI insight 2018 Oct 18;3(20)
JCI insight 2018 Oct 18;3(20)
Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination.
Nekrasova O, Harmon RM, Broussard JA, Koetsier JL, Godsel LM, Fitz GN, Gardel ML, Green KJ
Nature communications 2018 Mar 13;9(1):1053
Nature communications 2018 Mar 13;9(1):1053
Combined Mutation of Apc, Kras, and Tgfbr2 Effectively Drives Metastasis of Intestinal Cancer.
Sakai E, Nakayama M, Oshima H, Kouyama Y, Niida A, Fujii S, Ochiai A, Nakayama KI, Mimori K, Suzuki Y, Hong CP, Ock CY, Kim SJ, Oshima M
Cancer research 2018 Mar 1;78(5):1334-1346
Cancer research 2018 Mar 1;78(5):1334-1346
Multicolor quantitative confocal imaging cytometry.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes.
Pui HP, Saga Y
Mechanisms of development 2018 Feb;149:27-40
Mechanisms of development 2018 Feb;149:27-40
Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner.
O'Brien VP, Dorsey DA, Hannan TJ, Hultgren SJ
PLoS pathogens 2018 Dec;14(12):e1007457
PLoS pathogens 2018 Dec;14(12):e1007457
Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA.
Scavuzzo MA, Chmielowiec J, Yang D, Wamble K, Chaboub LS, Duraine L, Tepe B, Glasgow SM, Arenkiel BR, Brou C, Deneen B, Borowiak M
Cell reports 2018 Dec 26;25(13):3811-3827.e7
Cell reports 2018 Dec 26;25(13):3811-3827.e7
Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic α Cell Hyperplasia in Mice.
Kim J, Okamoto H, Huang Z, Anguiano G, Chen S, Liu Q, Cavino K, Xin Y, Na E, Hamid R, Lee J, Zambrowicz B, Unger R, Murphy AJ, Xu Y, Yancopoulos GD, Li WH, Gromada J
Cell metabolism 2017 Jun 6;25(6):1348-1361.e8
Cell metabolism 2017 Jun 6;25(6):1348-1361.e8
Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.
Molotkov A, Mazot P, Brewer JR, Cinalli RM, Soriano P
Developmental cell 2017 Jun 5;41(5):511-526.e4
Developmental cell 2017 Jun 5;41(5):511-526.e4
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection.
Treerat P, Prince O, Cruz-Lagunas A, Muñoz-Torrico M, Salazar-Lezama MA, Selman M, Fallert-Junecko B, Reinhardt TA, Alcorn JF, Kaushal D, Zuñiga J, Rangel-Moreno J, Kolls JK, Khader SA
Mucosal immunology 2017 Jul;10(4):1069-1081
Mucosal immunology 2017 Jul;10(4):1069-1081
A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease.
O'Brien VP, Hannan TJ, Yu L, Livny J, Roberson ED, Schwartz DJ, Souza S, Mendelsohn CL, Colonna M, Lewis AL, Hultgren SJ
Nature microbiology 2016 Oct 31;2:16196
Nature microbiology 2016 Oct 31;2:16196
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Ermilov AN, Kumari A, Li L, Joiner AM, Grachtchouk MA, Allen BL, Dlugosz AA, Mistretta CM
PLoS genetics 2016 Nov;12(11):e1006442
PLoS genetics 2016 Nov;12(11):e1006442
Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
Kato Y, Katsuki T, Kokubo H, Masuda A, Saga Y
Nature communications 2016 Apr 13;7:11272
Nature communications 2016 Apr 13;7:11272
E-cadherin can limit the transforming properties of activating β-catenin mutations.
Huels DJ, Ridgway RA, Radulescu S, Leushacke M, Campbell AD, Biswas S, Leedham S, Serra S, Chetty R, Moreaux G, Parry L, Matthews J, Song F, Hedley A, Kalna G, Ceteci F, Reed KR, Meniel VS, Maguire A, Doyle B, Söderberg O, Barker N, Watson A, Larue L, Clarke AR, Sansom OJ
The EMBO journal 2015 Sep 14;34(18):2321-33
The EMBO journal 2015 Sep 14;34(18):2321-33
AKT3 regulates ErbB2, ErbB3 and estrogen receptor α expression and contributes to endocrine therapy resistance of ErbB2(+) breast tumor cells from Balb-neuT mice.
Grabinski N, Möllmann K, Milde-Langosch K, Müller V, Schumacher U, Brandt B, Pantel K, Jücker M
Cellular signalling 2014 May;26(5):1021-9
Cellular signalling 2014 May;26(5):1021-9
Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion.
Ihermann-Hella A, Lume M, Miinalainen IJ, Pirttiniemi A, Gui Y, Peränen J, Charron J, Saarma M, Costantini F, Kuure S
PLoS genetics 2014 Mar;10(3):e1004193
PLoS genetics 2014 Mar;10(3):e1004193
Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells.
Dassah M, Almeida D, Hahn R, Bonaldo P, Worgall S, Hajjar KA
Journal of cell science 2014 Feb 15;127(Pt 4):828-44
Journal of cell science 2014 Feb 15;127(Pt 4):828-44
Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules.
Miyauchi E, Ogita T, Miyamoto J, Kawamoto S, Morita H, Ohno H, Suzuki T, Tanabe S
PloS one 2013;8(11):e79735
PloS one 2013;8(11):e79735
EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield JS
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):559-72
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):559-72
MMP20 modulates cadherin expression in ameloblasts as enamel develops.
Guan X, Bartlett JD
Journal of dental research 2013 Dec;92(12):1123-8
Journal of dental research 2013 Dec;92(12):1123-8
A critical role for TLR4 induction of autophagy in the regulation of enterocyte migration and the pathogenesis of necrotizing enterocolitis.
Neal MD, Sodhi CP, Dyer M, Craig BT, Good M, Jia H, Yazji I, Afrazi A, Richardson WM, Beer-Stolz D, Ma C, Prindle T, Grant Z, Branca MF, Ozolek J, Hackam DJ
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3541-51
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3541-51
N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs.
Toyama H, Arai F, Hosokawa K, Ikushima YM, Suda T
Biochemical and biophysical research communications 2012 Nov 23;428(3):354-9
Biochemical and biophysical research communications 2012 Nov 23;428(3):354-9
Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 1;188(5):2427-36
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 1;188(5):2427-36
Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
Betterman KL, Paquet-Fifield S, Asselin-Labat ML, Visvader JE, Butler LM, Stacker SA, Achen MG, Harvey NL
The American journal of pathology 2012 Dec;181(6):2225-38
The American journal of pathology 2012 Dec;181(6):2225-38
Noninvasive assessment of antenatal hydronephrosis in mice reveals a critical role for Robo2 in maintaining anti-reflux mechanism.
Wang H, Li Q, Liu J, Mendelsohn C, Salant DJ, Lu W
PloS one 2011;6(9):e24763
PloS one 2011;6(9):e24763
Effect of inhibition of vascular endothelial growth factor signaling on distribution of extravasated antibodies in tumors.
Nakahara T, Norberg SM, Shalinsky DR, Hu-Lowe DD, McDonald DM
Cancer research 2006 Feb 1;66(3):1434-45
Cancer research 2006 Feb 1;66(3):1434-45
FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
Grieshammer U, Cebrián C, Ilagan R, Meyers E, Herzlinger D, Martin GR
Development (Cambridge, England) 2005 Sep;132(17):3847-57
Development (Cambridge, England) 2005 Sep;132(17):3847-57
Multifunctionality of PAI-1 in fibrogenesis: evidence from obstructive nephropathy in PAI-1-overexpressing mice.
Matsuo S, López-Guisa JM, Cai X, Okamura DM, Alpers CE, Bumgarner RE, Peters MA, Zhang G, Eddy AA
Kidney international 2005 Jun;67(6):2221-38
Kidney international 2005 Jun;67(6):2221-38
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse E-Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse E-Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Cadherin in Mouse Intestinal Organoids. E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Cadherin in Mouse Intestinal Organoids. E-Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Cadherin in D3 Mouse Embryonic Stem Cell Line. E-Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Cadherin in Mouse Skin. E-Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- E-Cadherin in Mouse Spinal Cord. E-Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.