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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- F44156 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- E Cadherin Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 160-189 from the human protein was used as the immunogen for this E Cadherin antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the E Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of E Cadherin antibody and 293 lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of CDH1/ E Cadherin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using E Cadherin antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
