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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F53810 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- E-Cadherin Antibody / CDH1
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1579CT577.150.80
- Vial size
- 0.05 ml, 0.2 ml
- Storage
- Aliquot the E-Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431 and 2) T47D cell lysate with E-Cadherin antibody at 1:4000. Expected molecular weight: ~135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colon with E-Cadherin antibody at 1:25. HIER: steamed in pH6 citrate buffer.
