Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-3249-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton. Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers. E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided. The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells. Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of frozen tissue sections. Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of the MDCK cell line. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Canine
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- DECMA-1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT.
Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.
Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.
Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.
Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.
Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis.
Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis.
Reprogramming Roadblocks Are System Dependent.
Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer.
Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons.
Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer.
Single luminal epithelial progenitors can generate prostate organoids in culture.
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.
Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites.
Identification of a putative cell adhesion domain of uvomorulin.
Bornes L, van Scheppingen RH, Beerling E, Schelfhorst T, Ellenbroek SIJ, Seinstra D, van Rheenen J
Cell reports 2019 Nov 26;29(9):2565-2569.e3
Cell reports 2019 Nov 26;29(9):2565-2569.e3
Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.
Chua CW, Epsi NJ, Leung EY, Xuan S, Lei M, Li BI, Bergren SK, Hibshoosh H, Mitrofanova A, Shen MM
eLife 2018 Jan 15;7
eLife 2018 Jan 15;7
Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.
Ruetz T, Pfisterer U, Di Stefano B, Ashmore J, Beniazza M, Tian TV, Kaemena DF, Tosti L, Tan W, Manning JR, Chantzoura E, Ottosson DR, Collombet S, Johnsson A, Cohen E, Yusa K, Linnarsson S, Graf T, Parmar M, Kaji K
Cell stem cell 2017 Dec 7;21(6):791-805.e9
Cell stem cell 2017 Dec 7;21(6):791-805.e9
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.
Lyons-Cohen MR, Thomas SY, Cook DN, Nakano H
Journal of visualized experiments : JoVE 2017 Apr 5;(122)
Journal of visualized experiments : JoVE 2017 Apr 5;(122)
Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.
Jackson SA, Olufs ZP, Tran KA, Zaidan NZ, Sridharan R
Stem cell reports 2016 Mar 8;6(3):302-11
Stem cell reports 2016 Mar 8;6(3):302-11
Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.
Wuidart A, Ousset M, Rulands S, Simons BD, Van Keymeulen A, Blanpain C
Genes & development 2016 Jun 1;30(11):1261-77
Genes & development 2016 Jun 1;30(11):1261-77
Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis.
Ören B, Urosevic J, Mertens C, Mora J, Guiu M, Gomis RR, Weigert A, Schmid T, Grein S, Brüne B, Jung M
The Journal of pathology 2016 Jul;239(3):274-85
The Journal of pathology 2016 Jul;239(3):274-85
Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis.
Lovisa S, LeBleu VS, Tampe B, Sugimoto H, Vadnagara K, Carstens JL, Wu CC, Hagos Y, Burckhardt BC, Pentcheva-Hoang T, Nischal H, Allison JP, Zeisberg M, Kalluri R
Nature medicine 2015 Sep;21(9):998-1009
Nature medicine 2015 Sep;21(9):998-1009
Reprogramming Roadblocks Are System Dependent.
Chantzoura E, Skylaki S, Menendez S, Kim SI, Johnsson A, Linnarsson S, Woltjen K, Chambers I, Kaji K
Stem cell reports 2015 Sep 8;5(3):350-64
Stem cell reports 2015 Sep 8;5(3):350-64
Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer.
Zheng X, Carstens JL, Kim J, Scheible M, Kaye J, Sugimoto H, Wu CC, LeBleu VS, Kalluri R
Nature 2015 Nov 26;527(7579):525-530
Nature 2015 Nov 26;527(7579):525-530
Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons.
Wang S, Wang B, Pan N, Fu L, Wang C, Song G, An J, Liu Z, Zhu W, Guan Y, Xu ZQ, Chan P, Chen Z, Zhang YA
Scientific reports 2015 Mar 18;5:9232
Scientific reports 2015 Mar 18;5:9232
Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer.
Liang SQ, Marti TM, Dorn P, Froment L, Hall SR, Berezowska S, Kocher G, Schmid RA, Peng RW
Cell death & disease 2015 Jul 16;6:e1824
Cell death & disease 2015 Jul 16;6:e1824
Single luminal epithelial progenitors can generate prostate organoids in culture.
Chua CW, Shibata M, Lei M, Toivanen R, Barlow LJ, Bergren SK, Badani KK, McKiernan JM, Benson MC, Hibshoosh H, Shen MM
Nature cell biology 2014 Oct;16(10):951-61, 1-4
Nature cell biology 2014 Oct;16(10):951-61, 1-4
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Kaanta AS, Virtanen C, Selfors LM, Brugge JS, Neel BG
Breast cancer research : BCR 2013;15(4):R65
Breast cancer research : BCR 2013;15(4):R65
DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.
Oda M, Kumaki Y, Shigeta M, Jakt LM, Matsuoka C, Yamagiwa A, Niwa H, Okano M
PLoS genetics 2013 Jun;9(6):e1003574
PLoS genetics 2013 Jun;9(6):e1003574
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
O'Malley J, Skylaki S, Iwabuchi KA, Chantzoura E, Ruetz T, Johnsson A, Tomlinson SR, Linnarsson S, Kaji K
Nature 2013 Jul 4;499(7456):88-91
Nature 2013 Jul 4;499(7456):88-91
Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.
Kobayashi A, Donaldson DS, Kanaya T, Fukuda S, Baillie JK, Freeman TC, Ohno H, Williams IR, Mabbott NA
DNA research : an international journal for rapid publication of reports on genes and genomes 2012 Oct;19(5):407-22
DNA research : an international journal for rapid publication of reports on genes and genomes 2012 Oct;19(5):407-22
Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites.
Nakagawa M, Fukata M, Yamaga M, Itoh N, Kaibuchi K
Journal of cell science 2001 May;114(Pt 10):1829-38
Journal of cell science 2001 May;114(Pt 10):1829-38
Identification of a putative cell adhesion domain of uvomorulin.
Vestweber D, Kemler R
The EMBO journal 1985 Dec 16;4(13A):3393-8
The EMBO journal 1985 Dec 16;4(13A):3393-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the MDCK cell line with 0.125 µg of Rat IgG1 kappa Isotype Control Biotin (Product # 13-4301-82) (blue histogram) or 0.125 µg of Anti-Human CD324 (E-Cadherin) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
- Conjugate
- Biotin