- 710161 - Invitrogen Antibodies CDH1 antibody

Submitted references A simple and robust fluorescent labeling method to quantify trophoblast fusion.

Zhang Y, Yang H
Placenta 2019 Feb;77:16-18

Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis.

Roth B, Jayaratna I, Sundi D, Cheng T, Melquist J, Choi W, Porten S, Nitti G, Navai N, Wszolek M, Guo C, Czerniak B, McConkey D, Dinney C
Oncotarget 2017 May 23;8(21):34205-34222

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of E Cadherin in whole cell extracts of MCF-7 using an E Cadherin Recombinant Rabbit Polyclonal Antibody (Product # 710161) at a dilution of 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of E Cadherin in HEK293 cells using an E Cadherin Recombinant Rabbit Polyclonal Antibody (Product # 710161) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A, D) and actin stained with Alexa Fluor 594 phalloidin (red) (image B, E). Images C, F are composite images showing localization of E-cadherin at cell membrane and junction.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of E-Cadherin (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without E-Cadherin Recombinant Rabbit Polyclonal Antibody (Product # 710161), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of E-Cadherin/CD324 was performed on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with E-Cadherin/CD324 Recombinant Rabbit Polyclonal Antibody (Product # 710161) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody. The images were captured using a Nikon microscope at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Expression of epithelial and mesenchymal markers in vitro and in vivo ( A ) Comparison of epithelial markers (left panel) and mesenchymal markers (right panel) for UM-UC3 in vitro (2D culture) and in vivo after the 3rd generation of recycling. Gene expression by the primary tumor, CTCs, lymph node (LN) metastases, and distant metastases were characterized by qRT-PCR. ( B ) Relative mRNA expression of SNAIL in UM-UC3 following successive orthotopic tumor recycling. CTCs demonstrate increasing SNAIL expression with successive generations. ( C ) Comparison of epithelial markers (left panel) and mesenchymal markers (right panel) for UM-UC13 in vitro (2D culture) and in vivo after the 3rd generation of recycling using qRT-PCR. ( D ) Immunoblots for SNAIL and E-Cadherin of 2D culture cells, primary tumors, and metastases. ( E ) Cell suspension staining (GFP) for SNAIL in CTCs originating from mice with UM-UC3 orthotopic tumors which are luc-RFP labelled.

710161

Antibody data