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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- V3702 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant E-Cadherin Antibody / CDH1
- Antibody type
- Recombinant
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- ECD1-3R
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the recombinant E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human MCF-7 cell lysate with E-Cadherin antibody (clone ECD1-3R). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human cervix stained with recombinant E-Cadherin antibody (clone ECD1-3R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
