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Learn200-301-B19
antibody from Rockland Immunochemicals, Inc.
Targeting: AKT1
AKT, PKB, PRKBA, RAC, RAC-alpha
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 200-301-B19 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-B19, RRID:AB_1537387
- Product name
- Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-B19
- Antibody type
- Monoclonal
- Vial size
- 1 µl
Submitted references Distinctive subcellular Akt-1 responses to shear stress in endothelial cells.
Melchior B, Frangos JA
Journal of cellular biochemistry 2014 Jan;115(1):121-9
Journal of cellular biochemistry 2014 Jan;115(1):121-9
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunofluorescence confocal microscopy of Mouse Anti-AKT pS473 antibody. Tissue: EGF treated A431 cells. Fixation: 0.5% PFA. Antigen retrieval: EGF 15 min. Primary antibody: AKT pS473 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: DyLight 488 Goat anti-Rabbit IgG, MAb anti-AKT pS473, atto-647N anti-Mouse IgG (Active Motif). at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as red signal with tubulin (cyan).
- Sample type
- IF Microscopy
