Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ALR-021-50UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Latrophilin-1 (LPHN1) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (1:200).3,4. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody , preincubated with Latrophilin-1/LPHN1 (extracellular) Blocking Peptide (#BLP-LR021).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (1:200).3,4. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody , preincubated with Latrophilin-1/LPHN1 (extracellular) Blocking Peptide (#BLP-LR021).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Latrophilin-1 receptor in SH-SY5Y cell line - Cell surface detection of Latrophilin-1 receptor in intact living human neuroblastoma (SH-SY5Y) cells. A. Extracellular staining of cells with Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (red), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. DAPI is used as the counterstain (blue). C. Merged images of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Latrophilin-1 receptor in rat hippocampus - Immunohistochemical staining of rat hippocampal dentate granule layer (G) using Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021). A. Latrophilin-1 receptor staining (red) appears in astrocytes. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Latrophilin-1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (2.5μg) + goat- Anti-rabbit-FITC.