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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 200-301-D97 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-D97, RRID:AB_11183336
- Product name
- Anti-Norepinephrine Transporter (Mouse) Antibody - 200-301-D97
- Antibody type
- Monoclonal
- Description
- Anti-Norepinephrine Transporter antibody is directed against mouse Norepinephrine Transporter protein. The antibody was Protein G purified. Reactivity is expected from rat. Anti-NET shows no reactivity with tissue or cells from NET knock-out mice.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- NET-05
- Vial size
- 100 µl
- Concentration
- Titrated value sufficient to run approximately 10 mini blots. *
- Storage
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Mouse anti-Norepinephrine Transporter antibody. Lane 1: rat cortex homogenate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Norepinephrine Transporter antibody at 1:2,000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa for Norepinephrine Transporter. Other band(s): Norepinephrine Transporter splice variants and isoforms.
- Validation comment
- Western Blot
