BAF459

antibody from Novus Biologicals

Targeting: TNFRSF11B OCIF, OPG, TR1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF459

Provider

Novus Biologicals

Product name

Goat Polyclonal Osteoprotegerin/TNFRSF11B Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse RANK is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TGFβ-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis.

Li J, Ayoub A, Xiu Y, Yin X, Sanders JO, Mesfin A, Xing L, Yao Z, Boyce BF
Nature communications 2019 Jun 26;10(1):2795

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Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development.

McCarthy NI, Cowan JE, Nakamura K, Bacon A, Baik S, White AJ, Parnell SM, Jenkinson EJ, Jenkinson WE, Anderson G
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2675-82

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OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.

Shimamura M, Nakagami H, Osako MK, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 3;111(22):8191-6

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Cytokine-induced osteoprotegerin expression protects pancreatic beta cells through p38 mitogen-activated protein kinase signalling against cell death.

Schrader J, Rennekamp W, Niebergall U, Schoppet M, Jahr H, Brendel MD, Hörsch D, Hofbauer LC
Diabetologia 2007 Jun;50(6):1243-7

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Therapeutic relevance of osteoprotegerin gene therapy in osteosarcoma: blockade of the vicious cycle between tumor cell proliferation and bone resorption.

Lamoureux F, Richard P, Wittrant Y, Battaglia S, Pilet P, Trichet V, Blanchard F, Gouin F, Pitard B, Heymann D, Redini F
Cancer research 2007 Aug 1;67(15):7308-18

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Serotonin and fluoxetine modulate bone cell function in vitro.

Gustafsson BI, Thommesen L, Stunes AK, Tommeras K, Westbroek I, Waldum HL, Slørdahl K, Tamburstuen MV, Reseland JE, Syversen U
Journal of cellular biochemistry 2006 May 1;98(1):139-51

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17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.

Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood 2004 Sep 1;104(5):1404-10

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Osteoprotegerin/TNFRSF11B in Mouse Intestine. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF459) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.