Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- BAF805 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Osteoprotegerin/TNFRSF11B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant mouse Osteoprotegerin is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
Giovannini S, Tinelli G, Biscetti F, Straface G, Angelini F, Pitocco D, Mucci L, Landolfi R, Flex A
Cardiovascular diabetology 2017 Aug 8;16(1):99
Cardiovascular diabetology 2017 Aug 8;16(1):99
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
Cytokine 2006 Oct;36(1-2):57-68
Cytokine 2006 Oct;36(1-2):57-68
Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
Mandelin J, Li TF, Hukkanen M, Liljeström M, Salo J, Santavirta S, Konttinen YT
The Journal of rheumatology 2005 Apr;32(4):713-20
The Journal of rheumatology 2005 Apr;32(4):713-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Osteoprotegerin/TNFRSF11B in Human Prostate. Osteoprotegerin/TNFRSF11B was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.