Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB805-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Osteoprotegerin/TNFRSF11B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human Osteoprotegerin/TNFRSF11B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Osteoprotegerin is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/ TNFSF10-induced Cyto-toxicity and Neutral-ization by Human Osteoprotegerin/ TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (0.5 µg/mL), Recombinant Human Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 805-OS) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human TRAIL/ TNFSF10 (50 ng/mL) activity elicited by Recombinant Human Osteoprotegerin/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Osteoprotegerin/TNFRSF11B Monoclonal Antibody (Catalog # MAB805). The ND50 is typically 0.15-0.3 µg/mL.