A302-144A-T

antibody from Invitrogen Antibodies

Targeting: MADD DENN, KIAA0358, RAB3GEP

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-144A-T

Provider

Invitrogen Antibodies

Product name

MADD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MADD by immunohistochemistry. Sample: FFPE sections of human breast carcinoma and colon carcinoma. Antibody: Affinity purified rabbit anti-MADD (Cat. No. A302-144A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MADD by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MADD antibody A302-144A used for IP at 3 µg/mg lysate. MADD was also immunoprecipitated by rabbit anti-MADD antibody A302-143A, which recognizes an upstream epitope. For blotting immunoprecipitated MADD, A302-143A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.