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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-54055 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOV Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SCCLVCARQR GESCSDLEPC DESSGLYCDR SADPSNQTGI CTAVEGDNCV FDGVIYRSGE KFQPSCKFQC TCRDGQIGC Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOV in control (vector only transfected HEK293T lysate) and NOV over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NOV Polyclonal Antibody (Product # PA5-54055).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NOV in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a NOV Polyclonal Antibody (Product # PA5-54055).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOV in human adrenal gland using a NOV Polyclonal Antibody (Product # PA5-54055) shows strong cytoplasmic positivity in zona glomerulosa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOV in human skeletal muscle using NOV Polyclonal Antibody (Product # PA5-54055) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOV in human adrenal gland using NOV Polyclonal Antibody (Product # PA5-54055) shows moderate to strong cytoplasmic positivity in glandular cells.
