17548-1-AP

antibody from Proteintech Group

Targeting: HDAC8 HDACL1, KDAC8, MRXS6, RPD3, WTS

Provider product page for 17548-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [36]
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Validations
Western blot [1]

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Product number: 17548-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17548-1-AP, RRID:AB_2116926
Product name: HDAC8 antibody
Antibody type: Polyclonal
Description: KD/KO validated HDAC8 antibody (Cat. #17548-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HDAC8 protein structure - 17548-1-AP shown in red.

Submitted references Active compounds of licorice ameliorate microplastics-induced intestinal damage by targeting FADD.

Xue W, Fan X, Hui Y, Yu J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2025 Sep;203:115570

ZDHHC12 Palmitoylates HDAC8 to Promote the Progression of Hepatocellular Carcinoma Associated with a Diet High in Saturated Fatty Acids.

Jin X, Hong Y, Zhao Y, Shi W, Liu R, You X, Yang C, Zhang Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Oct;12(40):e05702

The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer.

Shang T, Jiang T, Tan J, Jiang H, Xu M, Pan Y, Lin Y, Cui X, Tian C, Feng H, Chen Y, Pei M, Geng X, Cheng S, Tan Y, Wang H, Dong L
Signal transduction and targeted therapy 2025 Oct 14;10(1):341

Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination.

Sun Y, Zhang WW, Men L, Wu J, Yao L, Huang X, Wu Y, Zhang C, Chen Y, Carling D, Zhang CS, Lin SC
Nature metabolism 2025 Nov;7(11):2324-2345

Epigenetic activation of PTEN by valproic acid inhibits PI3K/AKT signaling and Burkitt lymphoma cell growth.

Li C, Liu S, Gao J, Xu Y, Peng Q, Weng D, Wang D, Yang W, Yi P, Lin Z, Chen J
Gene 2025 May 20;950:149369

YAK577 Attenuates Cardiac Remodeling and Fibrosis in Isoproterenol-Infused Heart Failure Mice by Downregulating MMP12.

Zhou H, Kee HJ, Wan L, Asfaha Y, Fischer F, Kassack MU, Kurz T, Kim SH, Kee SJ, Hong YJ, Jeong MH
Korean circulation journal 2025 Mar;55(3):231-247

N(6)-Methyladenosine Regulates Cilia Elongation in Cancer Cells by Modulating HDAC6 Expression.

Rui Y, Zhang H, Yu K, Qiao S, Gao C, Wang X, Yang W, Asadikaram G, Li Z, Zhang K, Peng J, Li J, He J, Wang H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jan;12(2):e2408488

CK2B Induces CD8(+) T-Cell Exhaustion through HDAC8-Mediated Epigenetic Reprogramming to Limit the Efficacy of Anti-PD-1 Therapy in Non-Small-Cell Lung Cancer.

Liu S, Ma S, Liu G, Hou L, Guan Y, Liu L, Meng Y, Yu W, Liu T, Zhou L, Yuan Z, Pang S, Zhang S, Li J, Ren X, Sun Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(16):e2411053

Isoliquiritigenin ameliorates abnormal oligodendrocyte development and behavior disorders induced by white matter injury.

Wu D, Zhou W, Du J, Zhao T, Li N, Peng F, Li A, Zhang X, Zhang M, Hao A
Frontiers in pharmacology 2024;15:1473019

Inhibition of HDAC8 mitigates AKI by reducing DNA damage and promoting homologous recombination repair.

Wang Y, Yu C, Yu J, Shen F, Du X, Liu N, Zhuang S
Journal of cellular and molecular medicine 2024 Sep;28(18):e70114

HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC.

Qian K, Li W, Ren S, Peng W, Qing B, Liu X, Wei X, Zhu L, Wang Y, Jin X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Sep;11(36):e2401142

Targeting Nuclear Receptor Coactivator SRC-1 Prevents Colorectal Cancer Immune Escape by Reducing Transcription and Protein Stability of PD-L1.

Hong Y, Chen Q, Wang Z, Zhang Y, Li B, Guo H, Huang C, Kong X, Mo P, Xiao N, Xu J, Ye Y, Yu C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Sep;11(33):e2310037

A novel HDAC8 inhibitor H7E exerts retinoprotective effects against glaucomatous injury via ameliorating aberrant Müller glia activation and oxidative stress.

Wu LH, Cheng YW, Lin FL, Hsu KC, Wang MH, Yen JL, Wang TJ, Lin TE, Liu YC, Huang WJ, Hsiao G
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 May;174:116538

Probing class I histone deacetylases (HDAC) with proteolysis targeting chimera (PROTAC) for the development of highly potent and selective degraders.

Ibrahim HS, Guo M, Hilscher S, Erdmann F, Schmidt M, Schutkowski M, Sheng C, Sippl W
Bioorganic chemistry 2024 Dec;153:107887

Discovery of a Highly Potent and Selective HDAC8 Degrader: Advancing the Functional Understanding and Therapeutic Potential of HDAC8.

Xiao Y, Awasthee N, Liu Y, Meng C, He MY, Hale S, Karki R, Lin Z, Mosterio M, Garcia BA, Kridel R, Liao D, Zheng G
Journal of medicinal chemistry 2024 Aug 8;67(15):12784-12806

Proteolysis targeting chimera extracellular vesicles for therapeutic development treating triple negative breast cancer.

Erwin N, De U, Xiao Y, Wang L, Maharjan C, Pan X, Awasthee N, Zheng G, Liao D, Zhang W, He M
bioRxiv : the preprint server for biology 2024 Aug 26;

Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway.

Lan X, Lu M, Fang X, Cao Y, Sun M, Shan M, Gao W, Wang Y, Yu W, Luo H
Molecules (Basel, Switzerland) 2023 Sep 15;28(18)

HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation.

Xiao Y, Hale S, Awasthee N, Meng C, Zhang X, Liu Y, Ding H, Huo Z, Lv D, Zhang W, He M, Zheng G, Liao D
Cell chemical biology 2023 Nov 16;30(11):1421-1435.e12

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Wang T, Xu J, Xu Y, Xiao J, Bi N, Gu X, Wang HL
Cell reports 2022 Mar 8;38(10):110478

HDAC1 promotes the migration of human myeloma cells via regulation of the lncRNA/Slug axis.

Zheng L, Zhang A, Liu J, Liu M, Zhang Y
International journal of molecular medicine 2022 Jan;49(1)

Selective HDAC8 Inhibition Attenuates Isoproterenol-Induced Cardiac Hypertrophy and Fibrosis via p38 MAPK Pathway.

Zhao T, Kee HJ, Bai L, Kim MK, Kee SJ, Jeong MH
Frontiers in pharmacology 2021;12:677757

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Zhan W, Liao X, Liu J, Tian T, Yu L, Li R
Oncogenesis 2020 May 13;9(5):48

HDAC3 and HDAC8 are required for cilia assembly and elongation.

Park SA, Yoo H, Seol JH, Rhee K
Biology open 2019 Aug 9;8(8)

Stabilized Peptide HDAC Inhibitors Derived from HDAC1 Substrate H3K56 for the Treatment of Cancer Stem-Like Cells In Vivo.

Wang D, Li W, Zhao R, Chen L, Liu N, Tian Y, Zhao H, Xie M, Lu F, Fang Q, Liang W, Yin F, Li Z
Cancer research 2019 Apr 15;79(8):1769-1783

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Huang X, Yan J, Zhang M, Wang Y, Chen Y, Fu X, Wei R, Zheng XL, Liu Z, Zhang X, Yang H, Hao B, Shen YY, Su Y, Cong X, Huang M, Tan M, Ding J, Geng M
Cell 2018 Sep 20;175(1):186-199.e19

Curcumin Derivative Epigenetically Reactivates Nrf2 Antioxidative Stress Signaling in Mouse Prostate Cancer TRAMP C1 Cells.

Li W, Su ZY, Guo Y, Zhang C, Wu R, Gao L, Zheng X, Du ZY, Zhang K, Kong AN
Chemical research in toxicology 2018 Feb 19;31(2):88-96

The triterpenoid corosolic acid blocks transformation and epigenetically reactivates Nrf2 in TRAMP-C1 prostate cells.

Yang J, Wu R, Li W, Gao L, Yang Y, Li P, Kong AN
Molecular carcinogenesis 2018 Apr;57(4):512-521

Epigenetic modifications of triterpenoid ursolic acid in activating Nrf2 and blocking cellular transformation of mouse epidermal cells.

Kim H, Ramirez CN, Su ZY, Kong AN
The Journal of nutritional biochemistry 2016 Jul;33:54-62

Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.

Chen P, Yu N, Zhang Z, Zhang P, Yang Y, Wu N, Xu L, Zhang J, Ge J, Yu K, Zhuang J
Oncotarget 2016 Feb 2;7(5):6105-20

Epigenetics Reactivation of Nrf2 in Prostate TRAMP C1 Cells by Curcumin Analogue FN1.

Li W, Pung D, Su ZY, Guo Y, Zhang C, Yang AY, Zheng X, Du ZY, Zhang K, Kong AN
Chemical research in toxicology 2016 Apr 18;29(4):694-703

Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1.

Guo Y, Shu L, Zhang C, Su ZY, Kong AN
Biochemical pharmacology 2015 Mar 15;94(2):69-78

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Wang X, Liu J, Zhen J, Zhang C, Wan Q, Liu G, Wei X, Zhang Y, Wang Z, Han H, Xu H, Bao C, Song Z, Zhang X, Li N, Yi F
Kidney international 2014 Oct;86(4):712-25

Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications.

Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN
The AAPS journal 2014 Jul;16(4):727-35

Downregulation of histone deacetylase 1 by microRNA-520h contributes to the chemotherapeutic effect of doxorubicin.

Shen Q, Yao Q, Sun J, Feng L, Lu H, Ma Y, Liu L, Wang F, Li J, Yue Y, Jin H, Wang X
FEBS letters 2014 Jan 3;588(1):184-91

Epigenetic modifications of Nrf2 by 3,3'-diindolylmethane in vitro in TRAMP C1 cell line and in vivo TRAMP prostate tumors.

Wu TY, Khor TO, Su ZY, Saw CL, Shu L, Cheung KL, Huang Y, Yu S, Kong AN
The AAPS journal 2013 Jul;15(3):864-74

Epigenetic CpG demethylation of the promoter and reactivation of the expression of Neurog1 by curcumin in prostate LNCaP cells.

Shu L, Khor TO, Lee JH, Boyanapalli SS, Huang Y, Wu TY, Saw CL, Cheung KL, Kong AN
The AAPS journal 2011 Dec;13(4):606-14

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The HDAC8 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human HDAC8. This antibody recognizes human, mouse, rat antigen. The HDAC8 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 17548-1-AP.pdf

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