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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX113981 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113981, RRID:AB_2037142
- Product name
- HDAC8 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC8 antibody (GTX113981) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX113981 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HDAC8 antibody detects HDAC8 protein by Western blot analysis.A. 50 ?g rat heart lysate/extract10 % SDS-PAGEHDAC8 antibody (GTX113981) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX113981 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HDAC8 protein from 293T whole cell extracts using 5 £gg of HDAC8 antibody (GTX113981).Western blot analysis was performed using HDAC8 antibody (GTX113981).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. HDAC8 antibody (GTX113981) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HDAC8 antibody detects HDAC8 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HDAC8 antibody (GTX113981) diluted at 1:500.
