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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 685502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#685502, RRID:AB_2572204
- Product name
- Purified anti-HDAC8
- Antibody type
- Monoclonal
- Antigen
- Full length human HDAC8 recombinant protein expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W15090A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- BioLegend (provider)
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- Experimental details
- HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml purified anti-human HDAC8 (clone W15090A) antibody for one hour, followed by staining with Alexa Fluor® 594 anti-mouse IgG antibody (red), Alexa Fluor® 488 Phalloidin (green), and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
- Conjugate
- Unconjugated
