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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2336 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2336, RRID:AB_1575869
- Product name
- HDAC8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HDAC8.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning and characterization of a novel human class I histone deacetylase that functions as a transcription repressor.
Characterization of a highly complex region in Xq13 and mapping of three isodicentric breakpoints associated with preleukemia.
Cloning and characterization of human histone deacetylase 8.
Hu E, Chen Z, Fredrickson T, Zhu Y, Kirkpatrick R, Zhang GF, Johanson K, Sung CM, Liu R, Winkler J
The Journal of biological chemistry 2000 May 19;275(20):15254-64
The Journal of biological chemistry 2000 May 19;275(20):15254-64
Characterization of a highly complex region in Xq13 and mapping of three isodicentric breakpoints associated with preleukemia.
McDonell N, Ramser J, Francis F, Vinet MC, Rider S, Sudbrak R, Riesselman L, Yaspo ML, Reinhardt R, Monaco AP, Ross F, Kahn A, Kearney L, Buckle V, Chelly J
Genomics 2000 Mar 15;64(3):221-9
Genomics 2000 Mar 15;64(3):221-9
Cloning and characterization of human histone deacetylase 8.
Van den Wyngaert I, de Vries W, Kremer A, Neefs J, Verhasselt P, Luyten WH, Kass SU
FEBS letters 2000 Jul 28;478(1-2):77-83
FEBS letters 2000 Jul 28;478(1-2):77-83
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HDAC8 polyclonal antibody (Cat # PAB2336) in mouse NIH/3T3 cell lysate. HDAC8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the HDAC8 polyclonal antibody (Cat # PAB2336) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
