LS-B1452

antibody from LSBio

Targeting: PARK7 DJ-1, DJ1, GATD2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B1452

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B1452, RRID:AB_2160278

Product name

IHC-plus™ PARK7 / DJ-1 Antibody (C-Terminus) LS-B1452

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Zebrafish

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Human PARK7 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kD. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1300 dilution of the antibody overnight at 4C followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at RT. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.