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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP09257PU-N - Provider product page
- Provider
- OriGene
- Product name
- PARK7 (177-189) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PARK7 (177-189) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PARK7
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.3 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis is shown using anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,300 dilution of the antibody overnight at 4¡ã C followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT. IRDye(TM)800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry. Anti-PARK7 antibody was used at a 5 ug/ml to detect PARK7 in a variety of tissues. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows PARK7 staining of human pancreas. Tissue was formalinfixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
- Validation comment
- IHC
