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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB120-6619-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Carbonic Anhydrase I/CA1 Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.
