ABIN459009

antibody from antibodies-online

Targeting: CA1 Car1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

ABIN459009

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN459009, RRID:AB_10764401

Product name

anti-Carbonic Anhydrase I (CA1) antibody (Biotin)

Antibody type

Polyclonal

Antigen

Carbonic anhydrase I isolated and purified from human erythrocytes. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Description

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Isotype

IgG

Vial size

1 mL

Concentration

IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 4.9. No foreign proteins added.

Storage

The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is recons tituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.