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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00000759-M05-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Carbonic Anhydrase I/CA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CA1 - carbonic anhydrase I
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M05), clone M1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Detection limit for recombinant GST tagged CA1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Carbonic Anhydrase I/CA1 Antibody (M1) [H00000759-M05] - Analysis of CA1 transfected lysate using anti-CA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CA1 rabbit polyclonal antibody.
