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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-49698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous level of total Carbonic Anhydrase I / CA1 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ca1 was performed by loading Mouse liver tissue lysate (24µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Ca1 antibody (Product # PA5-49698) at a 1/450 dilution for 2 minutes, followed by Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ca1 was performed by loading Mouse liver tissue lysate (24µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Ca1 antibody (Product # PA5-49698) at a 1/450 dilution for 2 minutes, followed by Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution.
