Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C744791 - Provider product page
- Provider
- LSBio
- Product name
- CA1 / Carbonic Anhydrase I Antibody (Liquid) LS-C744791
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Goat anti-Carbonic Anhydrase I Antibody. Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of Goat anti-Carbonic Anhydrase I antibody. Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Carbonic Anhydrase I antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Carbonic Anhydrase 1 as precipitated red signal with hematoxylin purple nuclear counterstain.